Figure 5.

Albumin-cre mutants develop polycythemia secondary to elevated serum Epo levels. (A) Red blood cell (RBC) number and hemoglobin concentration in blood from 2-month-old mice were analyzed with a Hemavet counter. ●, Values for age-matched controls (total of six); ▴, values for albumin-cre mutants (total of six). (B) Raised serum Epo in albumin-cre mutants. A sandwich ELISA assay was used to measure serum Epo concentration. Mouse serum samples were diluted as indicated in 2% BSA and applied to the plates. Recombinant mouse Epo diluted in 2% BSA was used for the standard curve.