Table 1.

Forward and reverse primers (designed in Vector-NTI) and amplicon sizes for IFN-γ,^A IL-6,^B and the housekeeping gene β-actin are shown. RNA was detected by RT-PCR^C on lymphocytes derived from the HGs of chickens after AdCN94.H7 spray immunization.

Cytokine	Sense primer	Anti-sense primer	Amplicon
IFN-γ	GTCATTCAGATGTAGCTGACGGTGGA	CGCCATCAGGAAGGTTGTTTTTCA	201 bp
IL-6	GACGAGGAGAAATGCCTGACGAAG	CCACTTCATCGGGATTTATCACCATC	175 bp
β-actin	GCCATCTTTCTTGGGTATGGAGTCCT	GGTGGGGCAATGATCTTGATTTTCA	210 bp

^AIFN-γ = interferon γ.

^BIL-6 = interleukin 6.

^CRT-PCR = reverse transcriptase polymerase chain reaction.