Fig. 6.

AIV specific CD8⁺ T lymphocytes were detected from spleens of Ad-HA inoculated chickens 4 weeks p.b. Unseparated or the antibody enriched CD4⁺ and CD8⁺ splenic T lymphocytes isolated from chickens inoculated with adenovirus vector were stimulated ex vivo with MHC matched B19/B19 APCs infected with homologous H5N9 virus. Production of NO by macrophages induced by secretion of IFNγ from stimulated T lymphocytes was used to quantify the activation of the lymphocytes. Results are expressed as the average (± S.E.) of 4 birds. Each ex vivo stimulation assay is denoted by the adenovirus vector inoculated animal source of T lymphocyte and virus infected MHC-I APCs. AIV specific memory CD8⁺ T lymphocyte responses in the spleens were significantly greater than the CD4⁺ T lymphocyte responses (p≤0.003), but not significantly different from the response of the unseparated lymphocytes.