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. Author manuscript; available in PMC: 2011 Oct 15.

Published in final edited form as: Free Radic Biol Med. 2010 Jul 16;49(7):1221–1229. doi: 10.1016/j.freeradbiomed.2010.07.005

a. MEF were incubated with 5 μM CMH₂DCFDA in HBSS and stimulated with 1 μM PMA with or without tiron (10 mM) or cytochalasin-D (1 μM) as described in the Methods. Tiron-inhibitable fluorescent signal was significantly lower in the KO cells. Cytochalasin-D inhibited oxidants in the WT cells, but did not inhibit significantly in the KO cells. p < 0.05, p < 0.01. b. Macrophage-like RAW cells were treated with siRNA for Glrx-1 or control RNA for 2 days. PMA-induced oxidant generation was assessed by DCF assay. Oxidants generation was significantly inhibited in the cells treated with siRNA. p < 0.01. c. Western blot showed decreased expression of Glrx with siRNA.

a. MEF were incubated with 5 μM CMH₂DCFDA in HBSS and stimulated with 1 μM PMA with or without tiron (10 mM) or cytochalasin-D (1 μM) as described in the Methods. Tiron-inhibitable fluorescent signal was significantly lower in the KO cells. Cytochalasin-D inhibited oxidants in the WT cells, but did not inhibit significantly in the KO cells. p < 0.05, p < 0.01. b. Macrophage-like RAW cells were treated with siRNA for Glrx-1 or control RNA for 2 days. PMA-induced oxidant generation was assessed by DCF assay. Oxidants generation was significantly inhibited in the cells treated with siRNA. p < 0.01. c. Western blot showed decreased expression of Glrx with siRNA.