Figure 5.

Approach to implementing digital fluorescence lifetime sorting capabilities. In this figure, the darker boxed region depicts the low-cost and compact hardware necessary to retrofit a commercial sorting system, whose components are depicted by the lighter boxed region. A solid-state laser modulated with a function generator is placed in the commercial system’s optical path. Outputs from two PMTs are routed through high bandwidth preamplifiers and sent to ORCAS. The analog outputs are fed to the commercial system’s detector input.