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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Lung Cancer. 2010 Oct;70(1):28–36. doi: 10.1016/j.lungcan.2010.01.003

Fig. 3.

Fig. 3

Effect of hUCMSCs or IFN-β-hUCMSCs conditioned medium on the induction of apoptosis in H358 and SW1573 cell lines. H358 and SW1573 cells (3 × 105 cells/well) were seeded in 6-well plates. The conditioned media were applied 24 hrs after initial cell seeding. The cells were cultured for 24 hrs and the proportion of apoptotic cells was analyzed using Annexin V-FITC. A. Panels show the raw result of flow cytometry analysis of apoptosis in SW1573 cells incubated with various media for 24 hrs. B-i, control SW1573 cells cultured in un-conditioned medium; B-ii SW1573 cells cultured in hUCMSCs conditioned medium; B-iii SW1573 cells cultured in IFN-β-hUCMSCs conditioned medium; B-iv H358 cells cultured in hUCMSCs conditioned medium; B-v H358 cells cultured in IFN-β-hUCMSCs conditioned medium. The criteria for determining apoptosis of the cells are as follows: PI/Annexin-FITC = −/−, normal; PI/Annexin-FITC = −/+, early apoptotic (early); PI/Annexin-FITC = +/+, late apoptotic (late); PI/Annexin-FITC = +/−, damaged cells (damaged). Bar graphs indicate the average of two independent triplicate determinations.