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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Gastroenterology. 2010 Jun 25;139(3):1030–1040. doi: 10.1053/j.gastro.2010.05.007

Figure 3.

Figure 3

CD39 expression on bone marrow-derived cells facilitates tumor growth. Wild type (wt) and Cd39 null (null) chimeric mice were generated by bone marrow transplantation (BMT) (donor genotype designated first/recipients second). Eight weeks post-BMT, 1.5 × 105 luc-B16/F10 cells were infused via portal vein. (A) Tumor volumes on day 10 (n = 6–8 mice per group). (B) Representative images of tumor-bearing livers at day 10 (top) and in vivo bioluminescent imaging of tumor metastasis (bottom). (C) Representative immunohistochemical staining using anti-CD39 and anti-CD31 antibodies and Tunel staining (TdT) for apoptosis (Magnification x400 for CD39; x200 for CD31 and Tunel staining). (D) Ecto-ADPase activity in tumor-infiltrating mononuclear cells. Tumor-infiltrating mononuclear cells were isolated from tumor tissues of wt and Cd39 null tumor-bearing mice. Thin Layer Chromatography (TLC) analysis of ADP hydrolysis to AMP using 1.5 × 105 cells was analyzed. Data are given as means ± SEM. *P < .05.