Table 1.
Primary antibody
Antigen | Antibody | Concentration of dilution |
---|---|---|
Kir 4.1 (Chemicon International Inc., USA) amino acid residues 356–375 of rat, mouse or human Kir4.1 (Accession P49655)-synthetic peptide | Rabbit polyclonal | 4 µg IgG/ml |
Aquaporin 4 (AQP4) C-EKKGKDSSGEVLSSV (C-terminus) of the rat AQP4-synthetic peptide [7, 22] | Rabbit polyclonalSheep polyclonal | 5 µg IgG/ml5 µg IgG/ml |
α1-Syntrophin amino acid residues 491–505 (C-terminus) of rabbit α1-syntrophin-synthetic peptide [21, 24] | Rabbit polyclonalSheep polyclonal | 5 µg IgG/ml5 µg IgG/ml |
β-Spectrin (Transformation Research Inc., USA) extracted from human erythrocytes | Rabbit polyclonal | 1:100 |