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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: J Cell Biochem. 2010 Sep 1;111(1):207–217. doi: 10.1002/jcb.22690

Fig. 4.

Fig. 4

Identification of EGR2- (A.) and EGR3- (B.) responsive elements in the NAB2 promoter. Mel 888 and Colo 320DM cells were transfected with 100 ng of the indicated promoter reporter construct and 40 ng of human EGR2, EGR3, or PHLDA1 as control as described in “Experimental Procedures”. The results are presented as the mean and standard deviation of triplicates from a representative experiment. Left panel, results are presented as EGR2- or EGR3-mediated fold activity compared to PHLDA1 control plasmid (set as 1) of the indicated reporter constructs. Right panel, results are shown as percent of the maximum EGR2- or EGR3-mediated NAB2 promoter activty obtained with maluc-679, which was set as 100%. Before calculating the percentages the luciferase activity of the PHLDA1-transfected cells was subtracted from the luciferase activity of the NAB2 promoter constructs.