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. Author manuscript; available in PMC: 2011 Sep 1.

Published in final edited form as: J Cell Biochem. 2010 Sep 1;111(1):29–39. doi: 10.1002/jcb.22680

(A) Immunostaining was performed on BMEC cells to localize human EphB4 and ephrinB2 (rabbit polyclonal anti-human antibodies), using FITC-conjugated and PE-conjugated secondary antibodies, respectively. (B) RT-PCR analysis of EphB4 and ephrinB2 expression in endothelial cells compared to MEF. (C) EphB4−/− cells (400 cells) were induced to differentiate with (EphB4−/− and BMEC) or without (EphB4−/−) BMEC cells (200 cells) in hanging-drops. EphB4+/− ES cells were used as a control. The percentiles of beating EBs were quantified over time. Data are representative from 3 independent experiments. Error bars represent standard deviation.

(A) Immunostaining was performed on BMEC cells to localize human EphB4 and ephrinB2 (rabbit polyclonal anti-human antibodies), using FITC-conjugated and PE-conjugated secondary antibodies, respectively. (B) RT-PCR analysis of EphB4 and ephrinB2 expression in endothelial cells compared to MEF. (C) EphB4−/− cells (400 cells) were induced to differentiate with (EphB4−/− and BMEC) or without (EphB4−/−) BMEC cells (200 cells) in hanging-drops. EphB4+/− ES cells were used as a control. The percentiles of beating EBs were quantified over time. Data are representative from 3 independent experiments. Error bars represent standard deviation.