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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: J Virol Methods. 2010 Jun 25;169(1):39–46. doi: 10.1016/j.jviromet.2010.06.012

Figure 3.

Figure 3

Sensitivity to detect CXCR4-using variants within mixed viral populations. The results are the geometric mean RLU from multiple replicates with SE error bars. Minor species of X4-tropic env from HXB2 was detected consistently at 1% and approximately 50% of the times at 0.5%. Use of CXCR4 for entry was confirmed by ablation of the luciferase signal with the addition of AMD3100, which decreased the RLU to minimal levels. Luciferase activity in U87-CXCR4 cells decreased proportionally to the proportion of input DNA from X4 virus.