Skip to main content
. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Gastroenterology. 2010 May 24;139(3):904–917.e6. doi: 10.1053/j.gastro.2010.05.038

Figure 2. NOD2 activation limits TLR4 signaling in enterocytes both in vivo and in vitro.

Figure 2

A. IEC-6 cells or wild-type mice were treated with LPS±MDP as indicated. i: extent of NFkB activation IEC-6 cells as measured by ratio NFkB p65-subunit in nucleus vs. cytoplasm, ii: IL-6 release from IEC-6 cells by ELISA; iii: serum IL-6; iv: RT-PCR showing fold change in IL-6 in small intestinal mucosa. *p<0.05 vs. LPS alone in each case B: Confocal micrographs of IEC-6 cells corresponding to panel Ai immunostained for NFkB (green), actin(red), nucleus(blue), size bar=10μm. C i–iv: Confocal micrographs of IEC-6 cells treated as indicated and immunostained for TLR4 (green) and nuclei(blue); v: quantification of the surface expression of TLR4 using ImageJ software representative of 3 separate experiments. *p<0.05 vs. LPS alone.