Confocal analysis of the fraction volume of immunofluorescent NR1 in the anteroventral periventricular region. Fraction volume of NR1 does not change as a function of age (a) or steroid hormone treatment (b), nor is there an interaction of these variables (c). Hormone treatment groups are: VV, vehicle, vehicle; OV, 17β-oestradiol, vehicle; OP, 17β-oestradiol, progesterone. The numbers at the bottom of each bar represent the number of rats per group.