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The fraction volume of NR1 and NR2b colocalised in the AVPV increases with age (a) but not hormone treatment (b), and these two variables do not interact (c). Hormone treatment groups are: VV, vehicle, vehicle; OV, 17β-oestradiol, vehicle; OP, 17β-oestradiol, progesterone. The numbers at the bottom of each bar represent the number of rats per group. *P < 0.05, **P < 0.01.
