Fig. 6.
Caffeine dose-dependently reduces endoplasmic reticulum (ER) stress induced by cholesterol-enriched diet in rabbit hippocampus. Representative Western blots (a) and densitometric analysis (b) showing increased levels of the ER-resident proteins calreticulin, grp78 and grp94 and of the ER stress marker protein gadd153. Caffeine administered at 30 mg/day of caffeine/day in drinking water decreased the cholesterol-induced increase in calreticulin, grp78, grpP94, and gadd153 levels. At 0.5 mg/day, caffeine only reduces grp78 and calreticulin levels. *p<0.05, **p<0.01 versus controls, #p<0.05, # #p<0.01 versus rabbits fed with 2% cholesterol-enriched diet.