Figure 7.
A) Intensity coefficients of variance (CVs) for the peptides mixed with a 0.5 mg/mL human blood plasma sample at concentrations ranging from 1 nM to 1 μM. Mixing was performed prior to RP fractionations. Intensity CVs were calculated by integrating signals in the IMS domain. B) Number of identified unique peptides from human blood plasma as a function of the MP IMS-TOFMS experiment number. The peptides were matched against the detected IMS-TOF MS features at a mass measurement accuracy of 5 ppm and a normalized retention time tolerance of 4 %. The projected false discovery rate was estimated as ~ 4%.