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Immunocytochemical staining with phalloidin (red) showing expression and localization of F-actin in equine keratocytes, fibroblasts and myofibroblasts. Nuclei are stained blue by DAPI. Keratocytes (a) demonstrated no positive F-actin staining. Fibroblasts (b) were spindle shaped with intracellular F-actin bundles. Myofibroblasts (c) demonstrated strong F-actin staining showing prominent microfilament apparatus. Bar = 100 μm.
