Figure 1.

Chrm2 genotype and expression in inbred mouse strains. (A) Species and mouse strain comparisons of the amino acids surrounding P266L in CHRM2 (from http://www.ncbi.nlm.nih.gov/). An ID number is not available for BALB/cByJ mice, as this is the first identification of this sequence and it is not yet in the NCBI database. (B) The Sac II restriction enzyme and cDNA products of PCR amplification were used to create a marker for genotyping. The first lane on each gel represents a molecular weight ladder. The BALB/cByJ allele, which is resistant to the enzyme, is visible as a 451-bp band. The C57BL/6J allele is cleaved by the enzyme, resulting in a 392-bp fragment and a 59-base pair fragment that, due to its low weight, is not visible on the gel. Numbers 1 through 13 denote CXB recombinant inbred strains. The C57BL/6J allele is shared by strains C3H/HeJ and CXB strains 1, 2, 4, 11, 12, and 13. The BALB/cByJ allele is shared by strains A/J, DBA/2J, and CXB strains 3, 5, 6, 7, 8, 9, and 10. (C) Hypothalamus and basal forebrain were collected at 30 h after inoculation of mice with either uninfected allantoic fluid or allantoic fluid that contained influenza virus for measurement of Chrm2 mRNA. U, uninfected; I, influenza-infected, B6, C57BL/6J; C, BALB/cByJ.