Fig. 3.

Flow cytometric analysis of mitochondrial subpopulation ΔΨ_m in WT and db/db hearts. ΔΨ_m was assessed by staining mitochondrial subpopulations with JC-1 dye and assessing the shift from green to orange fluorescence with flow cytometry. A–D: representative histograms showing green and orange fluorescence in WT SSM (A) versus db/db SSM (B) and WT IFM (C) versus db/db IFM (D). E: ΔΨ_m was calculated based on orange-to-green fluorescence ratios in WT versus db/db cardiac mitochondrial subpopulations. Orange-to-green fluorescence ratios are expressed in AU. Values are means ± SE; n = 4 for each group. *P < 0.05 for WT vs. db/db.