Table 1.
Proteomic analysis of mitochondrial subpopulations from WT and db/db hearts
| SSMD/SSMC | IFMD/IFMC | SSMC/IFMC | |
|---|---|---|---|
| Mitochondrial fatty acid β-oxidation | |||
| Hydroxyacyl-CoA dehydrogenase | 1.08 | 1.21 | 1.26 |
| Glutamate oxaloacetate transaminase 2 | 1.13 | 0.74* | 0.75 |
| Mitochondrial trifunctional protein, β-subunit | 1.20 | 1.19 | NS |
| Acyl-CoA dehydrogenase, medium chain | 1.16 | NS | 0.80 |
| 3-Ketoacyl-CoA thiolase, mitochondrial | 1.24 | NS | NS |
| Acetyl-CoA acetyltransferase 1 | NS | 0.30* | 0.54 |
| Dihyrolipoamide dehydrogenase | NS | 0.75* | 0.85 |
| Dodecenoyl-CoA δ-isomerase | NS | 1.29 | NS |
| Mitochondrial respiratory chain | |||
| ATP synthase, H+-transporting mitochondrial F0 complex, subunit d | 0.90* | NS | 0.88 |
| ATP synthase H+-transporting F0 subunit F isoform 2 | 0.90* | 1.25 | 1.35 |
| Ubquinol cytochrome c reductase core protein 2 | 0.76* | NS | NS |
| Cytochrome b-c1 complex subunit 1 | NS | 1.44 | NS |
| Cytochrome c1, heme protein, mitochondrial | 0.85* | NS | 1.21 |
| Electron-transferring flavoprotein, β-polypeptide | 0.88* | 1.44 | 1.79 |
| Succinate dehydrogenase Fp subunit | NS | 0.68 | 0.66 |
| NADH dehydogenase (ubiquinone)-1α subcomplex 9 | NS | 1.16 | NS |
| NADH dehydrogenase (ubiquinone)-1β subcomplex 10 | 0.81* | NS | NS |
| NADH dehydrogenase (ubiquinone) Fe-S protein 6 | 0.70* | NS | 0.79 |
| NADH dehydrogenase ubiquinone FE-S protein 7 | 0.76* | NS | NS |
| Cytochrome c oxidase subunit VIc | 0.88* | 1.28 | 1.41 |
| Cytochrome c oxidase subunit Va | 0.75* | 1.20 | 1.75 |
| Cytochrome c oxidase subunit VIIa1 | 0.64* | NS | 0.82 |
| Cytochrome c oxidase subunit IV isoform 1 | NS | 1.16 | NS |
| Cytochrome c oxidase subunit VIb polypeptide 1 | NS | 0.81* | NS |
| Cytochrome c oxidase subunit II | NS | 1.32 | NS |
| Tricarboxylic acid cycle | |||
| Isocitrate dehydrogenase 2 (NADP+) | 1.12 | 1.23 | NS |
| Isocitrate dehydrogenase 3 (NAD+), α | NS | 1.28 | 1.35 |
| Malate dehydrogenase 2, NAD | NS | 0.79* | NS |
| Succinyl-CoA ligase [ADP-forming] subunit-β, mitochondrial | NS | 1.30 | |
| Amino acid metabolism | |||
| Dihydrolipamide S-succinyltransferase | NS | 0.61* | NS |
| Oxidative stress related | |||
| Peroxiredoxin V | 0.80* | NS | NS |
| Transport proteins | |||
| Carrier family 25 (mitochondrial carrier, phosphate carrier), member 3 | 1.15 | 0.87* | 1.17 |
| Glucose-related protein 75, mitochondrial heat shock protein70 | 0.80* | NS | 1.26 |
| Solute carrier family 25 (mitochondrial carnitine/acylcarnitine translocase), member 20 | NS | 1.28 | 0.64 |
| Voltage-dependent anion-selective channel 2 | 1.24 | NS | 0.67 |
| Solute carrier family 25 (adenine nucleotide translocator), member 4 | 0.80* | 1.28 | NS |
| Carnitine O-palmitoyltransferase 1, muscle isoform | NS | 1.37 | 0.647 |
| Voltage-dependent anion-selective channel 1 | NS | 0.70* | 0.77 |
| Voltage-dependent anion-selective channel 3 | NS | 0.50* | NS |
| Miscellaneous | |||
| NipSnap homolog 2 | NS | 1.46 | 1.36 |
| Es 1 protein | 1.15 | 0.67* | 0.85 |
| Elongation factor tu, mitochondrial | NS | 0.46* | 0.73 |
| Aldehyde dehydrogenase | 0.69* | NS | NS |
| Mirq-like protein | NS | 1.21 | NS |
| Desmin | 0.66* | NS | NS |
n = 4 for each group. Shown is the iTRAQ analysis of identified and significantly changed proteins, which were categorized into groups consisting of fatty acid oxidation, tricarboxylic acid cycle, mitochondrial respiratory chain, amino acid-related, oxidative stress, transport, and miscellaneous proteins in isolated subsarcolemmal mitochondria (SSM) and interfibrillar mitochondria (IFM) from wild-type [WT; control (C)] and db/db [diabetic (D)] hearts. Treatment differences are indicated ratiometrically, with positive changes unmarked and negative changes marked with an asterisk (*). NS, no significant differences between any group; all other values were significant (P < 0.05 for WT vs. db/db).