Fig. 1.

Vorinostat induces accumulation of acetylated α-tubulin, histone H3, and γH2AX in normal (HFS) and transformed (LNCaP, A549) cells. Cells were cultured with 5 μM vorinostat for indicated times. Immunoblots are of acetylated α-tubulin (A), acetylated histone H3 and γH2AX (B), immunoblots of γH2AX and acetylated histones H3 and H4 at early time points (C), GAPDH, and total histone H3 are indicated as the loading controls. Graphs, expressed as a ratio to the loading control, of the accumulation of acetylated α-tubulin (D), acetylated histone H3 (E), and γH2AX (F). Graphs were prepared by quantifying immunoblot bands by using TINA 2.0 software (Ray Tests).