Skip to main content
. 2010 Aug 2;107(33):14627–14632. doi: 10.1073/pnas.1004302107

Fig. 3.

Fig. 3.

In vivo sampling and tissue-based molecular diagnostics with STAMP methodology. (A) Sampling of IgG from mouse skin was investigated using different surfactant formulations. While sampling with conventional surfactants (TritonX-100 and SLS) was unable to represent the actual concentration of IgGs in skin, STAMP sampled IgG at a concentration that was statistically indistinguishable from the native IgG concentration in skin. (B and C) Sampling of diagnostic biomarkers localized in tissue microenvironment was investigated using a mouse model of allergic dermatitis. STAMP rapidly sampled about 3-fold higher amount of allergy biomarkers (IgE antibodies; B) from eczematic skin than healthy mouse skin (p < 0.05). No statistically significant difference was found between the amounts of IgG antibodies in the STAMP samples from allergic and healthy mice skin (C). Cotton swabbing, performed as a control procedure, sampled significantly lower amounts of IgE and IgG antibody than STAMP (p < 0.05) and could not differentiate between healthy and allergic mouse skin. Errors bars indicate SD N = 4.