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. 2010 Aug 2;107(33):14745–14750. doi: 10.1073/pnas.1001562107

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Fig. 1. — Identification of the CX₃CR1⁺ CD8α⁺ DC subset. (A) Flow cytometric analysis of splenocytes from Cx₃cr1^gfp/+ mice. cDC were identified as CD11c^hi cells. Histogram shows GFP expression in CD11c^hi CD8α⁺ DC. (B) Staining of splenic cDC subpopulations for surface expression of CX₃CR1 using a CX₃CL1-Fc fusion protein. (C) Flow cytometric analysis of lymph node cells from Cx₃cr1^gfp/+ mouse. (D) Flow cytometric analysis of splenocytes from Cx₃cr1^g^fp/+ BALB/c mouse. (E) Flow cytometric analysis of splenocytes from CX₃CR1-deficient Cx₃cr1^gfp/gfp mice. (F) Flow cytometric analysis of splenocytes from Cx₃cr1^gfp/+ mouse. Note characterization of CX₃CR1/GFP⁺ CD8α⁺ DC as a CD86^lo CD103^neg population.