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Denaturant-dependent collapse and charge-mediated expansion of unfolded proteins. Dependence of the mean transfer efficiencies, 〈E〉, for CspTm (yellow), IN (red), and ProTαC (blue) on the concentration of GdmCl (filled circles) and urea (open circles). The typical uncertainty in transfer efficiency of individual data points is in the range of 0.02. Error bars are shown for conditions where multiple measurements are available.
