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E-IgM-iC3b binding assay. (A) Binding of E-IgM-iC3b to αxβ2 in the presence of the same combinations of Fabs as used in EM. Mn2+ (1 mM) was used as a positive control. *, P < 0.01. (B) Effect of TS1/18 Fab concentration on binding in the presence of CBR LFA-1/2 Fab. (C) Effects of individual Fabs on E-IgM-iC3b binding. Background binding of ~255 cells/mm2 of E-IgM-iC3b to the BSA blocked surface has been subtracted.
