Fig. 5.

Time course of relative expression levels of defense-related genes in soybean suspension cells in response to GmSubPep as analyzed by real-time RT-PCR. RNA was isolated from suspension cells at various times after addition of either GmSubPep or a control peptide at a final concentration of 25 nM as described in Materials and Methods. The gene expression levels are indicated relative to expression at 0 h. Cyp93A1, a cytochrome P450 gene involved in phytoalexin synthesis; Chib-1b, chitinase gene, a pathogenesis-related gene; PDR12, a salicylic acid–inducible ATP-binding cassette transporter; achs, chalcone synthase, involved in phytoalexin production. ELF1B was amplified as an internal control to normalize the RNA level in each sample. Error bars indicate SD from three biological replicates.