Figure 1.

Expression of recombinant rat SR-BI in Sf9 insect cells infected with recombinant baculoviruses encoded for this protein. (Upper) Cell lysates (20 μg) prepared from cells 24, 36, 48, 60, 72, or 96 h postinfection subjected to 7% SDS/PAGE and immunoblotted with anti-SR-BI. The immunoreactive bands were detected by chemiluminescence. (Lower) Infected control cells (A) or SR-BI-expressing cells (B) immunostained for SR-BI, examined by confocal fluorescence microscopy, and subsequently color adjusted by using photoshop techniques. SR-BI-expressing cells showed patchy expression of the SR-BI protein (pink).