Fig. 3.

The effect of long-term denervation on overloaded muscles studied ex vivo. Number of nuclei counted (A) and CSA measured on isolated EDL muscle fibers (B) taken from muscle before the onset of overload (Control Pre), after 14 days of overload (Overload), after 14 days of overload and the subsequent 2 mo of denervation (Overload + Denervation), after 3 mo of denervation (Denervation), and from normal muscles from the same batch of animals that had not been exposed to experimental treatment (Control Post). Each column represents the mean ± SEM of 134–378 fibers. Columns that were statistically indistinguishable (P > 0.05) are marked with the same letter.