Fig. 4.

miR771 is a new siRNA trigger. (A) Predicted foldback of MIR771_. The expected duplexes of miR771 and miR771* are highlighted. (B) Construct of 35S:771FP and cleavage sites identified by 5′ RACE assay. The position of the cleavage site is indicated by an arrow with the proportion of sequenced clones. FP-siR probe (horizontal line) indicates a DNA oligonucleotide probe complementary to the 42-nt region downstream of the predicted cleavage site directed by miR771 for the detection of FP-derived siRNAs shown below. (C) Northern blot analysis of miR771, FP-derived siRNA, and 771FP transcripts.