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. 2010 Aug 9;107(34):15281–15286. doi: 10.1073/pnas.1009056107

Fig. 2.

Fig. 2.

AtsPLA2-α represses AtMYB30 transcriptional activity. Transient transactivation of the KCS1p by AtMYB30 in N. benthamiana leaves. Lanes: 1,KCS1p:GUS reporter alone or with: 2, AtMYB30-TAP; 3, AtMYB30-TAP+AtsPLA2-α-HA; 4, AtMYB30-TAP+AtsPLA2-α-H62Q-HA; 5, AtMYB30-TAP+AtsPLA2-β-HA; 6, AtMYB30-TAP+AtsPLA2-γ-HA; 7, AtMYB30-TAP+AtsPLA2-δ-HA. (A) Fluorimetric GUS assays in leaf discs 36 hpi. Mean and SEM values are calculated from eight independent experiments (two to four replicates per experiment). MU, methylumbelliferone. (B) Western blot showing expression of AtMYB30-TAP and HA-tagged AtsPLA2 constructs. Ponceau S staining confirms equal loading.