Table 1.

FLIM analysis shows that AtMYB30 interacts with AtsPLA₂-α in the nucleus of N. benthamiana epidermal cells

Donor	Acceptor	Lifetime*	SD	n†	E‡	P value§
AtMYB30-CFP	—	2.306	0.030	60	—	—
AtMYB30-CFP	AtsPLA2-α-YFPv	2.067	0.024	46	10.4	4.0 x 10−8
AtMYB30-CFP	AtsPLA2-α-H62Q-YFPv	2.053	0.031	20	10.9	2.1 x 10−5
AtMYB30-CFP	AtMYB123-YFPv	2.278	0.041	31	—	0.58
AtMYB30ΔMYB-CFP	—	2.345	0.064	30	—	—
AtMYB30ΔMYB-CFP	AtsPLA2-α-YFPv	2.323¶	0.071	20	—	0.82

*Mean lifetime in nanoseconds.

^†Total number of measured nuclei.

^‡FRET efficiency percentage (E = 1 − τDA/τD) was calculated by comparing the lifetime of the donor in the presence of the acceptor (τDA) with its lifetime in the absence of the acceptor (τD).

^§P value of the difference between the donor lifetimes in the presence and in the absence of the acceptor (Student's t test).

^¶Values were measured in areas outside the nucleus where AtsPLA₂-α-YFPv and AtMYB30ΔMYB colocalize.