Fig. 4.

Effects of increased H₂O₂ induced by CR or by inactivation of CTA1 on oxidative damage to macromolecules. (A) Oxidative damage was assessed by measuring levels of oxidized proteins (carbonyls) in stationary phase wild-type and Δcta1 cells under non-CR and CR conditions. Levels of carbonyls were normalized at each time point to wild-type cell values under non-CR conditions (2% glucose). (B) Oxidative damage to proteins and lipids measured as autofluorescence of stationary phase wild-type and Δcta1 cells under non-CR and CR conditions. Histograms are representative of data collected at day 3. Values indicate mean ± SEM from three independent experiments. Statistical significance (*P < 0.05) was determined by Student's t-test.