Fig. 5.

Characterization of WT and mutant N-ABD aggregates. (A) EM picture, (B) CD spectra of aggregates. (C) Second derivative FTIR spectra of aggregates (dashed curves) compared to the soluble WT monomer (solid curve). (D) Increased ThT fluorescence when bound to protein aggregates. The figure shows the fluorescence in buffer before and after adding aggregates. (E) Red shift in CR absorption spectra upon binding to protein aggregates. The figure shows the spectra before and after adding aggregates. In panels B–E, individual curves were not labeled because the minor differences in intensity can be accounted by experimental errors. (F) Congo red birefringence images of dystrophin N-ABD fibrils.