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. 2010 Aug 9;107(34):15081–15086. doi: 10.1073/pnas.1009575107

Fig. 2.

Fig. 2.

Estrogen enhances and antiestrogens disrupt the ERα–p53 interaction on the p21 promoter, leading to diametrically opposite effects on transcription of p21 compared with that of pS2. (A) qChIP was performed to analyze the ERα–p53 interaction on the p21 promoter in MCF-7 cells. Cells were grown in media with dextran-coated charcoal-treated FBS for 4 d and treated with E2 (10 nM) with or without 1 μM 4-hydroxy tamoxifen or ICI 182780 for 3 h. (B) Western analysis of ERα and p53 protein levels in lysates of MCF-7 cells treated with E2, E2 plus tamoxifen, or E2 plus ICI 182780. (C and D) The effects of estrogen and antiestrogens on p21 and pS2 transcription, respectively, in MCF-7 cells, as assayed by qPCR (value = mean ± SD; *P < 0.05; ***P < 0.001).