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. 2010 Aug 9;107(34):15081–15086. doi: 10.1073/pnas.1009575107

Fig. 3.

Fig. 3.

Differential effects of ERα bound to ERE-containing promoters versus p53 binding site-containing promoters. (A) ChIP using anti-p53 or anti-ERα antibodies to analyze p53 and ERα binding to endogenous p21 and pS2 gene promoters in MCF-7 cells treated with E2, E2 plus tamoxifen, or E2 plus ICI 182780. (B) ChIP assay using anti-NCoR antibody, demonstrating differential recruitment of NCoR to pS2 vs. p21 gene promoters in MCF-7 cells treated as in A. (C) ChIP assay using SRC1 and SRC3 antibodies, demonstrating differential recruitment of SRC1 and SRC3 to pS2 versus p21 gene promoters in MCF-7 cells treated as in A. (D) A model for the dual roles of ERα in promoting proliferation via direct binding to ERE elements and indirect binding to p53 binding sites (by tethering to p53).