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. Author manuscript; available in PMC: 2011 Aug 31.
Published in final edited form as: J Immunol Methods. 2010 Jun 30;360(1-2):119–128. doi: 10.1016/j.jim.2010.06.017

Figure 3. Flow cytometry analysis of monocyte populations.

Figure 3

First, monocytes were gated based on FSC and SSC (A). Doublets (B), dead cells (C), CD3+ T and CD20+ B lymphocytes (D) were excluded. HLA-DR+ and CD14+ cells were selected (E) and three monocyte subsets were gated based on expression of CD14 and/or CD16. These include the classical monocytes (CD14+CD16−) and activated monocytes (CD14+CD16+ and CD14−CD16+) (F). Contamination from granulocytes that are HLA-DR- and CD14dim are excluded from monocyte analysis, based on HLA-DR and CD14 expression (Figure 3E, black arrow). Results presented here are from one animal and are representative of n=12. Numbers are percentages of each population within the same dotplot.