. 2010 Aug 12;10:88. doi: 10.1186/1471-213X-10-88

Table 2.

The effects of microinjection of different substrates into the cytoplasm of one-cell stage fertilized eggs on early embryo developmental potential

Different substrate treatment	Total embryo treated in one-cell stage^†	No.(%) of survival embryo after treatment	No.(%) of embryos developed to

			2-cell embryo	4-cell embryo	8-cell embryo	Morula embryo	Blastocyst embryo
2 mM granzyme G Morpholino	80	43(53.8)	39(90.7)^a	5(11.6)^a	0(0.0)^a	0(0.0)^a	0(0.0)^a
2 mM control Morpholino	90	45(50.0)	45(100.0)^ab	42(93.3)^b	42(93.3)^b	37(82.2)^b	26(57.8)^b
M2 buffer injection	46	20(43.5)	20(100.0)^ab	17(85.0)^b	17(85.0)^bc	17(85.0)^b	17(85.0)^c
Control embryo (Uninjected)	74	--	74(100.0)^b	70(94.6)^b	57(77.0)^c	57(77.0)^b	57(77.0)^bc

^†Six experiments were carried out in each group, and 10-15 embryos were used in each experiment.

^abcDifferent superscripts within a column indicate a statistically significant difference (p < 0.05).