Table 3.

The developmental potential of mouse embryos cultured in vitro after microinjection of different concentrations of granzyme G antisense morpholinos into the cytoplasm of one-cell stage fertilized eggs

Dosage of Granzyme G Morpholino	Total embryo treated in one-cell stage†	No. (%) of survival embryo after treatment	No. (%) of embryos developed to
			2-cell embryo	4-cell embryo	8-cell embryo	Morula embryo	Blastocyst embryo
2 mM	64	31(48.4)	25(80.7)a	7(22.6)a	0(0.0)a	0(0.0)a	0(0.0)a
0.2 mM	65	33(50.8)	32(97.0) b	29(87.9)b	25(75.8)b	24(72.7)b	11(33.3)b
0.02 mM	81	45(55.6)	45(100.0)b	45(100.0)c	43(95.6)c	43(95.6)c	43(95.6)c
0.002 mM	63	39(61.9)	38(97.4)b	36(92.3)bc	34(87.2)bc	34(87.2)bc	34(87.2)c

^† Six experiments were carried out in each group, and 10-15 embryos were used in each experiment.

^abc Different superscripts within a column indicate a statistically significant difference (p < 0.05).