FIGURE 6.
Transition density plots depict the remodeling of the free energy landscape due to mutation in tRNA binding sites. Plotting the initial and final FRET values of each transition indicates the existence of three distinct FRET states corresponding to the classical state and two hybrid states. The distribution of transitions shifts as a result of alterations in the energy landscape of the ribosome-tRNA complex due to removal of ribosomal protein L1 (A), or mutations made in key rRNA residues (B–D).