Fig. 12. The Same Cell-Restricted Complex Forms on an Q50 Homeodomain Consensus Site and an rGnRH CAATTA Repeat.
EMSA with radiolabeled Q50 homeodomain consensus site (Q50, lanes 5–8) and −1642/−1623 probes (lanes 1–4) with GT1–7 nuclear extract. Molar excess (100-fold) of unlabeled oligonucleotide was included for competition as follows: −1642/−1623, lanes 2 and 7; GnRH promoter CAATTA repeat region −63/−33, lanes 3 and 8; Q50 homeodomain consensus binding site, lanes 4 and 6. An arrow indicates the cell-restricted complex.