FIGURE 8.

Whole-cell patch-clamp analysis of the M8M2-nud chimera. A, illustration of the channel structure of the M8M2-nud chimera, in which the NUDT9 domain of TRPM2 was C-terminally linked to TRPM8 (amino acids 1097–1104 of TRPM8 were replaced with amino acids 1168–1503 of TRPM2). B, whole-cell currents of a cell expressing M8M2-nud. The pipette solution contained 1 mm ADPR and 1 μm Ca²⁺. After infusion of ADPR and Ca²⁺ for >2 min did not induce currents, the cell was exposed to ice-cold bath solution for ∼10 s (as indicated by the horizontal black bar) and then stimulated with the menthol analog WS-12 (30 μm; arrow). The example shown is representative of five similar experiments. C, I-V relation of currents shown in B obtained during voltage ramps from −150 to +150 mV from a holding potential of −60 mV. RT, room temperature.