FIGURE 4.

Qualitative growth assay of yeast strains harboring different Mal phenotypes on maltose, isomaltose, and αMG. The wild-type CEN.PK113–7D strain (Mal⁺) and its MAL2–8^c deletion mutant, the wild-type BY4741 strain (Mal⁻), and the BY4741 strain transformed with a CEN plasmid bearing the constitutively expressed MAL2–8^c allele of the MAL23 gene, were grown on YN liquid medium containing 1% sugar (maltose, isomaltose, or αMG). The cultivation tubes were incubated at 30 °C for 24 h prior to being photographed.