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. 2010 Jun 18;285(35):26815–26824. doi: 10.1074/jbc.M110.145946

TABLE 2.

List of primers used for qPCR analysis

Forward (F) and reverse (R) primer sequences and PCR amplification efficiency (Eff) are shown.

ORF Primer sequence Eff.
%
MAL12 (id. to MAL32) F: GGCGTTGATGCTATTTGGGTTTGT 99
R: GAGGTCTCCAGAAGAACCAGTCAC
YGR287c/IMA1 F: CGATGCCATTTGGATCTCACCATTC 88
R: ACCAGTCACGCTTTGGATTAGTCTTC
YOL157c/IMA2 F: GACTTAGTCATCAACCATTGCTCG 85
R: ACAGTCCTCATTCTCCCAATTCAA
YIL172c/IMA3 (id. to YJL221c/IMA4) F: GACTTAGTCATCAACCATTGCTCC 92
R: GCAGTCTTCGTTCTCCCAGTTTAG
YJL216c/IMA5 F: ACAATGACGGATGGGGTGATTTAGC 90
R: CAACAATAACCTTGATACCTCTCTTATGAGC