TABLE 2.
τ and ΔR(0) parameters and distance, rP-e, of the diC6PC 31P to spin-label sites of D205C and H82C extrapolated from the slower correlation time
Parameter | D205C | H82C |
---|---|---|
τf (ns) | 13.0 ± 9.1 | 6.8 ± 1.3 |
ΔRf(0) (s−1) | 0.17 ± 0.07 | 0.040 ± 0.005 |
τs (ns) | 112 ± 11 | 65.1 ± 3.3 |
ΔRs(0) (s−1) | 0.93 ± 0.07 | 0.15 ± 0.01 |
rP-e (Å) | 12.7 ± 0.2a | 15.7 ± 0.1a |
a Both τs and ΔRs(0) could be obtained in the fit for both samples (5 mm diC6PC and 14.4 μm protein), with slightly larger errors in τs. Therefore, the maximum and minimum τs were used to find the range in rP-e (and hence the error).