FIGURE 5.

The slc4a10 polypeptide mediates Cl⁻ efflux but not Cl⁻ influx. A, mean rate constants for the ³⁶Cl⁻ efflux of mouse slc4a10-transfected and control cells during the first 2 min after readdition of Na⁺ to acidified cells in the absence (BBS) and the presence of DIDS. The difference in apparent rate constants between the columns for each cell line represents the Na⁺-dependent DIDS-sensitive change in Cl⁻ per time unit (n = 5). B, similar experiments performed on cells transfected with slc4a10 in presence/absence of CO₂/HCO₃⁻ and DIDS. Rate constants were normalized to the value obtained in the absence of HCO₃⁻. These ³⁶Cl⁻ efflux measurements were run in parallel with slc4a7-transfected cells (C) and dsRed-transfected cells (D). In separate experiments, ³⁶Cl⁻ influx was measured for the first 2 min of pH_i recovery after acidification in cells transfected with slc4a10 (E) or slc4a7 (F) in the presence/absence of CO₂/HCO₃⁻. *, statistical significance.