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. 2010 Jun 15;285(35):27033–27044. doi: 10.1074/jbc.M110.120352

FIGURE 6.

FIGURE 6.

Single channel analysis of CFTR constructs K584E-, L581F-, and L581F/K584E-CFTR. A, representative recordings of CFTR Cl− channels in excised inside-out membrane patches from BHK cells expressing the indicated CFTR variants cultured at 37 °C. ATP (1 mm) and protein kinase A (75 nm) were continuously present in the intracellular solution, voltage was −50 mV, and there was a large Cl− concentration gradient across the membrane patch ([Cl−]int = 147 mm; [Cl−]ext = 10 mm). Dotted lines indicate where channels are closed, and downward deflections correspond to channel openings. For WT-, F508del-, K584E-, and L581F/K584E-CFTR, membrane patches contained a single active channel, but for L581F-CFTR, the membrane patch contained two active channels. B and C, single channel current amplitude (i) and Po of the indicated CFTR variants recorded using the conditions described in A. Data are means ± S.E. (n = 4–5 except F508del-CFTR where n = 10). Asterisks indicate values that are significantly different from those of wild-type CFTR (p < 0.05). In C, Po values for F508del-CFTR are from Ref. 24. Error bars in B and C correspond to S.E.