FIGURE 4.

FEN1 mutants localize to the telomere. A, FEN1 alleles localize to the telomere. Representative ChIP analysis of 293T cells (Ctrl) or 293T cells transfected with wild-type FEN1 (hWT or mWT) or FEN1 mutants (ΔP, ΔPΔC, or mED), subjected to immunoprecipitation with the FLAG (M2) antibody. Precipitated DNA was probed for the presence of telomeric sequences as described under “Experimental Procedures.” The inputs indicate 0.1 and 0.2% of total. B, quantification of the representative ChIP assay is shown. Percent of telomeric DNA immunoprecipitated with the FLAG antibody was calculated using input DNA, and the control pulldown percentage was set to 1.