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Detection of NqrA, NqrB, and NqrC in membranes containing NQR subcomplexes. Washed membranes from V. cholerae Δnqr transformants expressing different sets of Nqr subunits were solubilized with SDS and membrane proteins (30 μg) were separated by SDS-PAGE. FMN covalently bound to subunits NqrB and NqrC was detected by in gel fluorography (A and B). NqrA was detected by Western blot analysis against its N-terminal His6 tag (C).
