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Flavin analysis of membranes containing NQR subcomplexes. Washed membrane vesicles (∼1 mg of protein) from V. cholerae Δnqr transformants expressing different sets of Nqr subunits were analyzed for flavins by HPLC. Elution of flavins was monitored at 450 nm. Elution times of standards (FAD, 120 pmol; FMN, 37 pmol; riboflavin, 56 pmol) are indicated.
