FIGURE 7.

Determination of the mass of holo-Na⁺-NQR by static light scattering. The analysis was performed online with Na⁺-NQR eluting from a Superdex 200 column. The UV absorbance signal (thin solid line) and the differential refractive index signal (thin dashed line) are shown. Na⁺-NQR eluted at 21.94 min. The calculated molar masses of the detergent fraction (thick light gray line, bottom), the protein fraction (thick dark gray line, middle), and the protein-detergent conjugate (thick black line, top) are shown.